Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown

Journal: Virology

Article Title: Zika virus infection of adult and fetal STAT2 knock-out hamsters.

doi: 10.1016/j.virol.2017.04.013

Figure Lengend Snippet: Fig. 2. ZIKV immunohistochemistry of testes of STAT2 KO hamsters s.c. injected with ZIKV. ZIKV immunofluorescence (mouse monoclonal antibody to ZIKV NS1 protein) of testes from ZIKV-infected hamsters #01 (A-C,E-G), #61 (I-K), and sham (M-O) at 10 dpi, and ZIKV-specific peroxidase (mouse anti-flavivirus group antigen) catalyzed staining of Vero cells co-cultivated with homogenized testes from ZIKV-infected hamster #01 (Q) and sham (R). ZIKV-specific immunoperoxidase staining counterstained with hematoxylin of testes tissue (D,H,L,P). Solid arrow head – spermatogonia morphology; Solid arrow – Sertoli cell morphology. Scale bar =50 µM.

Article Snippet: Either mouse anti-flavivirus group antigen monoclonal antibody (Millipore, CA) or mouse monoclonal antibody to ZIKV NS1 protein (Aalto Bio Reagents, Dublin, Ireland) were incubated on the tissues for overnight.

Techniques: Immunohistochemistry, Injection, Infection, Staining, Immunoperoxidase Staining

Journal: Virology

Article Title: Zika virus infection of adult and fetal STAT2 knock-out hamsters.

doi: 10.1016/j.virol.2017.04.013

Figure Lengend Snippet: Fig. 4. ZIKV and histopathology of placental tissues from STAT2 KO hamsters s.c. injected with 500 CCID50 of ZIKV 8 dpc. Tissues were collected at 14 dpc (6 dpi). ZIKV immunofluorescence (mouse monoclonal antibody to ZIKV NS1 protein) using peroxidase-catalyzed staining counter-stained with hematoxylin (A-E). Scale bar =50 µM.

Article Snippet: Either mouse anti-flavivirus group antigen monoclonal antibody (Millipore, CA) or mouse monoclonal antibody to ZIKV NS1 protein (Aalto Bio Reagents, Dublin, Ireland) were incubated on the tissues for overnight.

Techniques: Histopathology, Injection, Staining